The prognostic potential and oncogenic effects of PRR11 expression in hilar cholangiocarcinoma

题目：《The prognostic potential and oncogenic effects of PRR11 expression in hilar cholangiocarcinoma 》（译：《PRR11 在肝门部胆管癌中的表达及其对预后的影响》）

摘要：PRR11 is a newly identified oncogene in lung cancer, yet its role in others tumors remains unclear. Gastrointestinal tissue microarrays were used

to evaluate PRR11 expression and its association with clinical outcome was analyzed in patients with hilar cholangiocarcinoma. Overexpression of PRR11 was

observed in esophageal, gastric, pancreatic, colorectal, and hilar cholangiocarcinoma. Expression of PRR11 correlated with lymph node metastasis and CA199 level in two HC patient cohorts. After an R0 resection, a high level of PRR11 expression was found to be an independent indicator of recurrence (P = 0.001). In cellculture, PRR11 silencing resulted in decreased cellular proliferation, cell migration, tumor growth of QBC939 cells. Microarray analysis revealed that several genes involved in cell proliferation, cell adhesion, and cell migration were altered in PRR11-knockout cells, including: vimentin (VIM), Ubiquitin carboxyl-terminal hydrolase 1 (UCHL1), early growth response protein (EGR1), and System A amino acid transporter1 (SNAT1). Silencing PRR11 inhibited the expression of UCHL1, EGR1, and SNAT1 proteins, with immunoassays revealing a significant correlation among the levels of these four proteins. These results indicate that PRR11 is an independent prognostic indicator for patients with HC.（译：PRR11是肺癌中新发现的癌基因，但其在其他肿瘤中的作用尚不清楚。应用胃肠道组织芯片检测PRR11在肝门部胆管癌患者中的表达，并分析其与临床预后的关系。在食管、胃、胰腺、大肠和肝门部胆管癌中观察到PRR11的过度表达。两组HC患者PRR11表达与淋巴结转移及CA199水平相关。R0切除后，高水平的PRR11表达被发现是复发的独立指标（P=0.001）。在细胞培养中，PRR11沉默导致QBC939细胞增殖、细胞迁移和肿瘤生长减少。微阵列分析显示PRR11敲除细胞中参与细胞增殖、细胞粘附和细胞迁移的几个基因发生改变，包括：波形蛋白（VIM）、泛素羧基末端水解酶1（UCHL1）、早期生长反应蛋白（EGR1）和系统A氨基酸转运蛋白1（SNAT1）。沉默PRR11可抑制UCHL1、EGR1和SNAT1蛋白的表达，免疫分析显示这四种蛋白水平之间存在显著相关性。这些结果表明PRR11是HC患者的独立预后指标。）